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KMID : 0606920110190020195
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Biomolecules & Therapeutics
2011 Volume.19 No. 2 p.195 ~ p.200
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Cytoprotective Effects of Docosyl Cafferate against tBHP-Induced Oxidative Stress in SH-SY5Y Human Neuroblastoma Cells
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Choi Yong-Jun
Kwak Eun-Bee
Lee Jae-Won
Lee Yong-Suk
Cheong Il-Young
Lee Hee-Jae
Kim Sung-Soo
Kim Myong-Jo
Kwon Yong-Soo
Chun Wan-Joo
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Abstract
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Neuronal cell death is a common characteristic feature of a variety of neurodegenerative disorders including Alzheimer’s disease and Parkinson’s disease. However, there have been no effective drugs to successfully prevent neuronal death in those diseases. In the present study, docosyl cafferate (DC), a derivative of caffeic acid, was isolated from Rhus vernicifl ua and its protective effects on tBHP-induced neuronal cell death were examined in SH-SY5Y human neuroblastoma cells. Pretreatment of DC significantly attenuated tBHP-induced neuronal cell death in a concentration-dependent manner. DC also signifi cantly suppressed tBHP-induced caspase-3 activation. In addition, DC restored tBHP-induced depletion of intracellular Bcl-2, an anti-apoptotic member of the Bcl-2 family. Furthermore, DC signifi cantly suppressed tBHP-induced degradation of IKB, which retains NF-kB in the cytoplasm, resulting in the suppression of nuclear translocation of NF-kB and its subsequent activation. Taken together, the results clearly demonstrate that DC exerts its neuroprotective activity against tBHP-induced oxidative stress through the suppression of nuclear translocation of NF-kB.
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KEYWORD
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Docosyl cafferate (DC), SH-SY5Y human neuroblastoma cells, tBHP, Apoptosis, NF-kB
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